Euphorbia hirta has been widely used by Tribal as traditional medicine in a treatment against infectious pathogens. E. hirtaLinn. is a perennial. diffusion method was used to determine the antibacterial activity on gram negative: Euphorbia hirta plant displayed an excellent antibacterial activity against K. The antibacterial and antifungal effect of Euphorbia hirta was evaluated against a panel of bacteria (Escherichia coli, Psueudomonas aeruginosa, Klebsiella.

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Quantifying the bioactivity of the plant extracts during screening and bioassay guided fractionation. Footnotes Source of Support: Antimicrobial potency of flavonoids free and bound of E.

Selected extract bound flavonoid of stem which showed activity against all microorganisms tested was dissolved in ethyl acetate and applied on silica gel coated 0. Euphorbia hirta belonging to family Euphorbiaceae is a medicinal, rhizomatous herb antkmicrobial in southern Western Ghats of India and northern east coast of Tamil Nadu. Pharmacological activities of Chelidonium majus L. Int Eupnorbia Appl Sci Eng. Screening for antimicrobial activity Disc diffusion assay was performed for antimicrobial screening.

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Please review our privacy policy. Pharmacognostical and antibacterial studies of leaf extracts of Euphorbia hirta L. Broth microdilution method was followed for determination of MIC values. In all test microbes most susceptible organism in the investigation was C. Eight pathogenic microorganisms in total, including four bacteria, viz.

Financial assistance provided by UGC is or acknowledged. TA indicates the volume at which extracts can be diluted without losing the ability to kill microorganisms. Different parts root, stem, leaf, and fruits of E. Out of the four bacteria tested, S. Free and bound flavonoid extract exhibited good inhibitory activity against most of the pathogens. Subramanian SS, Eu;horbia S. The filtrate was extracted in ethyl acetate and washed with distilled water to neutrality. Present investigation proves the plant under study to possess broad spectrum antimicrobial activity.

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An international multicenter study of antimicrobial consumption and resistance in Staphylococcus aureus isolates from 15 hospitals in 14 countries. Bonjar G, Farrokhi PR. Nil Conflict of Interest: In Nigeria, extracts or exudates of the plant are used as ear drops and in the treatment of boils, sore, and promoting wound healing. The isolated compounds were crystallized, weighed, and subjected to melting point m.

Antimicrobial activity of essential oil extracts of various onions Allium cepa and garlic Allium sativum Lebensm-Wiss-U-Technol. This article has been cited by other articles in PMC.

Antimicrobial activity of flavonoids free and bound of Euphorbia hirta L. Two flavonoids quercetin and kaempferol were identified in the bound flavonoids of stem extract which showed activity against all the microorganisms. Table 1 Rf values of spots of flavonoid extract obtained in TLC solvent system benzene: Geeta Singh and Padma Kumar.

The present investigation evaluates the antibacterial and hitta effects of free and bound flavonoids of E. Free and bound flavonoids from root, stem leaf, and fruits of E.

In the current investigation E.

Most of the extracts showed high values of TA against P. Antibacterial and antifungal activity was expressed in terms of activity index AI. Antibacterial activity of flavonoids of Euphorbia hirta by disc diffusion assay. Antimicrpbial 6 Quantity and total activity of free and bound flavonoids of Euphorbia hirta. In the present investigation lowest MIC value 0.

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Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta

Flavonoids in genotypes of Trifolium subterraneum. Free flavonoids of stem showed bioactivity against P. Extraction procedure Plant parts were separately shade dried and finely powdered using a mixer.

Open in a separate window. Preparative TLC of the bound flavonoids from stem of E. TA and quantity of extracts from plant parts was calculated and recorded [ Table 6 ]. The turbidity of the wells in the microtiter plate was interpreted as visible growth of microorganisms. Antibacterial activity of selected Seaweads from Kovalan south West coast of India.

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All eight extracts showed significant antimicrobial activity against test microbes. The growth of fungi A. Results of the present investigation indicate that E. Each dilution was added to broth media of wells of microtiter plates.

Out of fungi; A. Results of the present study reveals that all the eight tested plant extracts inhibit the growth of selected bacteria and fungi; indicating broad spectrum bioactive nature of selected plant. Wong E, Francis CM. J Med Plant Res. Proteomic approach to understanding antibiotic action.